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doi: 10.1016/j.scitotenv.2020.142458 154 LiangYLiuDZhanJLuoMHanJWangPet al

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If outside these windows, wait until the next regularly scheduled dose

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Avoid repeated freeze-thaw cycling, and verify concentration by UV absorbance at 280 nm where assay design requires it, since the single tryptophan residue gives a usable chromophore