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PMID: 41739483, PMCID: PMC12936964, DOI: 10.1001/jamasurg.2025.6859

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Selective covalent modification of KRAS G13C In mass spectrometry (MS) studies with KRAS G13C 1169 (Cys-light), we observed covalent modification of the desired cysteine 13 for all compounds and that the reactivity increased with rising pH levels, which can be attributed to the enhanced nucleophilicity of the cysteine

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The results suggested that the additional amino acids had little effect on the enzyme activity

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