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Bottom line: Higher NAD+ = better energy, sharper cognition, and cellular longevity

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Because GHK-Cu interacts with regenerative, collagen-related, antioxidant-support, and tissue-maintenance pathways, observed responses may reflect broader physiological-resilience and recovery-focused signaling mechanisms associated with these pathways

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After blocking with 5% nonfat dry milk in TBS-Tween for 12 h at room temperature, blots were incubated with appropriate primary antibodies overnight at 4 C