ipamorelin effect on gh pulse frequency Effects of a Growth Hormone-Releasing Hormone Analog Endogenous Pulsatility and Insulin Sensitivity in Healthy Men ipamorelin effect on gh pulse

Levison, Ph.D., Robert A

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D.OuryT.RabouilleC.SlotJ

Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)

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