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FFA and glycerol were quantified on the Cobas 6000 analyser in samples that were supplemented with sodium fluoride for stabilization

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Insert the needle into the vial, turning it upside downt, and inject the air into the vial

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( C ) ESR spectra of DEPMPO-trapped radicals, recorded from HeLa cells expressing APEX2 in either the cytosol (upper left trace) or the mitochondrial matrix (upper right trace), after exposure to HPI (1 mM), H 2 O 2 (1 mM) and DEPMPO (50 mM)