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pH 7.4) and 50% of (20% ACN, 0.1% FA) were kept at 8 C prior to analysis of 2 L of pure compound mixtures or reacted extracts by the autosampler into a 15 L flow-through needle

Compared to the active state, ECL1 moves toward helix I by 5 when measured at the N-terminal tip of the helix (C of Q211), and ECL1 residue W214 is reoriented ~180 o from an outside-facing position to a position pointing towards helix I

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