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Description

In brief, for the HITI donor vector containing the sgAlb-E14 expression cassette and scExe4 donor fragment, the sgAlb-E14 expression cassette and scExe4 (NGF-FCS2-Exe4) sequence flanked by two sgAlb-E14 gRNA target sequences were prepared by PCR using PrimeSTAR GXL polymerase (Takara Bio)

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This not only may lead to treatment interruption but also may increase the risk of malnutrition and dehydration caused by reduced intake [64]

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