In the identification of unknown compounds, the use of high-resolution hybrid quadrupole (Q)-TOF, additionally coupled with high performance liquid chromatography (LC-QTOF-MS/MS) is irreplaceable (Johansson et al., 2014
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After SDSPAGE, the following antibodies were used: CDK11 (rabbit antiserum, 1:3000), P-T235-SF3B1 (rabbit antiserum, 1:3000), GST (Santa Cruz, sc-138, 1:200), U2AF65 (Santa Cruz, sc-53942, 1:400), RBM17 (Sigma, HPA037478, 1:1000)
Gomes 2014
Notably, C57BL/6 male mice that received sulforaphane in conjunction with depression-inducing lipopolysaccharide, exhibited lower depressive behaviours on the tail-suspension test and forced swimming test compared to those that did not receive sulforaphane (Zhang et al., 2017)
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