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Further exploration demonstrated that Swell1 overexpression in a mouse microglial cell line, BV2 cells, cultured in a hypotonic environment to activate chlorine channels, activated cAMP response element-binding protein (CREB) and forkhead box O3 (FOXO3a) transcription factors and the negative regulator of the NLRP3 inflammasome, WNK lysine deficient protein kinase 1 (WNK1) (Chen et al., 2023)

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qPCR validation analyses TotalRNA from each tissue was aliquoted and frozen at -80 C, and 2 g of total RNA was reverse-transcribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Catalog # 4368813)

A greater decrease in thyroxine and triiodothyronine was found, compared to the poisoned group

The 10-Si material still had 25% lower water absorption than 10-CuBG, but both materials remained below the ISO recommended 40 g/mm 3 for water sorption, unlike 10-BG

Furthermore, the effects of quercetin were noticeable from the first week of treatment, where a significant decrease in the ocular itching sensation score was observed in the active group 1 week after the start of ingestion of the food supplement ( p = 0.02) [72]