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Furthermore, a lower dose of NAC (20 mm) did not increase mucosal GSH levels and failed to reduce the injury of TNBS+ethanol-treated rats, further supporting that the protection of a high dose of NAC against TNBS+ethanol induced damage was related to the increased GSH levels

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Then, the NPT ensemble was further employed for 50 ns to equilibrate the system at 300 K and 1 atm, followed by a 10 ns production run for collecting equilibrated configurations at each 10 ps interval