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This process is closely related to improve the secretion of intestinal L cell and islet cell, activate GLP-1R/PKA signaling pathway through autocrine and paracrine, and increase the expression of its related molecule such as GLP-1, GLP-1R, PC1/3, PC2, PKA, Pdx1

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Density gradient centrifugation was used to extract chloroplasts of Arabidopsis or soybean according to the manufacturers instructions of the Chloroplast Extraction Kit (PTE010, Coolaber)