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The reaction mixture (1 ml) containing Lockes buffer (pH 7.4), 0.2 ml homogenate and 10 l of DCFH-DA (5 mM) was incubated for 45 min at room temperature and the fluorescence intensity of DCF was recorded by excitation at 485 nm and emission at 535 nm using a Synergy TM 2 Multi-function Microplate Reader (Bio-Tek Instruments Inc., Winooski, Vermont, USA)

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Patient data, including diagnoses, laboratory test results, and reports, were extracted for patients admitted to AMC from January 1, 2000, to December 30, 2020

Furthermore, supplementation with riboflavin results in marked lowering of homocysteine concentrations specifically in individuals with the variant MTHFR 677TT genotype (15)

Follicular fluid IGF concentrations correlate with oocyte competence