They cause damage to vessels through by increasing the number of thrombins, causing NOX activation and elevated production of ROS, which leads to DNA and lipid oxidation, concurring to atherosclerosis [57], which has also been confirmed in Jagadeesha et al.s [44] research
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The next experimental step included extracting total RNA from the cells through the use of the acid guanidinium thiocyanate-phenol-chloroform extraction method
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