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Based on these results, we proposed a Funnel model incorporating both assays for efficient early-stage screening of BBB-permeable compounds (Figure 4)

Comparative example 2: 7-8Fmoc-Ala-Thr (pro-me-me) -OH, 21-22Fmoc-Ser-Ser (pro-me-me) -OH preparation of Cagrilintide peptide resin: the 7 th-8 th amino acid of the Cagrilintide sequence is used as pseudo-prolyl dipeptide Fmoc-Ala-Thr (pro-me-me) -OH, and the 21 st-22 th amino acid of the Cagrilintide sequence is used as pseudo-prolyl dipeptide Fmoc-Ser-Ser (pro-me-me) -OH Weighing 2.85g of Rink AmideAM resin (resin substitution degree of 0.35mmol/g) into the solid phase reactor, and adding 50mL of DCM to swell the resin for 0.5 h

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Small amounts of alcohol are generally tolerated by oily skin or people who live in humid environments