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Proc Natl Acad Sci Usa 2020;117:1914150

Summary: Hydrolyzing Amides To Carboxylic Acids With Acid Or Base Acidic hydrolysis of amides is one of those meat and potatoes reactions of chemistry that are essential to know and understand

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Live-cell microscopy RAW 264.7 macrophages were seeded in Nunc 8-well Lab-Tek chambered coverglass (ThermoFisher) at 23 10 5 cells per well in 500 l complete minimum essential medium (MEM, Gibco) supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 C with 5% CO 2 for 16 h

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Nitrous oxide inactivates vitamin B12 in your body and makes it so your cells cannot use it to complete important processes