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(2013) 8:e68322

doi: 10.1046/j.1600-079X.2003.00092.x 102

This means being transparent about where our ingredients come from and how they are formulated

Sample type considerations Different biological matrices have different detection characteristics: Urine: Most common testing matrix, metabolites accumulate Blood/plasma: Shorter detection windows for most peptides Dried blood spots: Emerging method with good sensitivity Hair: Not commonly used for peptides but theoretically possible Most anti-doping testing uses urine

Primary antibodies were diluted in TBST skimmed milk 5% buffer and the incubation was performed overnight at 4C using an orbital shaker followed by 2 h at room temperature with the secondary horseradish peroxidase (HRP)-linked antibody (Goat Anti-Mouse or Anti-Rabbit IgG Peroxidase Conjugated by Thermo Scientific)