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To further determine whether GLP-1R and PKA signalling were involved in the regulation of metformin functions in HUVECs, the protein levels of GLP-1R and phosphorylated PKA (p-PKA) were examined

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Flow cytometry analysis (Supplementary Fig

However, as GLP-1s expand into broader therapeutic categories, as new molecular analogues and combination regimens enter development pipelines, and as payers and regulators begin demanding evidence beyond empirical efficacy alone, this scientific blind spot is quietly becoming one of the most consequential constraints facing the entire sector