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Reductase enzymatic activity The reductase activities of the WT and mutant (K8A, C20A and K47A) proteins were measured spectrophotometrically by recording the increase in absorbance at 260 nm using a UV-VIS spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan) as described previously 37

doi: 10.1371/journal.pone.0172536 22

Standard L-glutathione has very poor oral bioavailability compared to liposomal versions

Yes, because the most effective way to quickly restore glutathione levels is with a course of liposomal glutathione, the most bioavailable form for the body

Next, serum-free medium was used when the cells reached 70% of confluence

Ferroptosis as a P53-mediated activity during tumour suppression