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10.1016/S0140-6736(17)32284-5 26

GMO-free and free from 14 allergens: Sunflower lecithin as the sole carrier, without soy, gluten, lactose or animal-derived ingredients

HCT116 Flp-in cells (seeded in 10 cm plates, 30% confluency) were co-transfected with 9 g of donor plasmid and 3 g of sgRNA-Cas9 plasmid, and 24 l Turbofect (Thermo Scientific, R0533) in 2 ml Opti-MEM (Thermo Scientific, 11058-021)

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DNA dot-blot analysis Genomic DNA from erythroblasts was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated with ethanol before dissolving in water