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Although the combined treatment did not further ameliorate Hb level and reticulocyte count (Supplemental Figure 10, A and B), it produced a greater change in the Hb to RDW ratio, a marker for spherocytes and dense RBCs (Figure 6D), and further decreased annexin-V positivity of RBCs (Figure 6E)

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Importantly, GSH, the main low molecular weight intracellular thiol antioxidant, effectively induced cyclization of AX

Consistent with this, exendin-P5, which induces faster kinetics in G protein activation and cAMP production, exhibits higher cAMP efficacy than GLP-1 and exendin-4, as indicated by a greater pEC 50 /pIC 50 ratio when comparing cAMP and binding studies 13