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After 1.5 g of residue was dissolved in 10 mL of methanol, 0.15 mL of hydrazine monohydrate was added, and this mixture was refluxed at 80C for 1 h

The assay includes a control test tube to ensure the accuracy of the GPx activity measurements and address potential interferences from other thiols and sample components

In modern peptide protocols, CJC-1295 is typically paired with ipamorelin (a different mechanism) rather than sermorelin (the same mechanism)

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