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Lead-in calorie restriction enhances the weight-lowering efficacy of incretin hormone-based pharmacotherapies in mice

While this effect is often considered beneficial in research contexts, it necessitates careful tracking to prevent too much glucose lowering, very in subjects with already normal insulin response

RNA extraction and RT-qPCR Total RNA was extracted from each sucrose gradient fraction using Trizol reagent (Invitrogen)

This finding may be attributed to the limitations associated with small sample sizes in clinical research

doi:10.1002/14356007.a02_269.pub2

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