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The assay mixture containing 50 mM sodium phosphate buffer (pH 7.8) and hypoxanthine 50 M and the cytosol fraction (50 uL) was incubated at 37C for 5 min

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Hence, by targeting the enzymatic activity of SARM1, inhibitors may effectively prevent the depletion of NAD + , thereby maintaining cellular energy homeostasis and enhancing neuronal resilience, potentially expanding the treatment landscape for various neurological conditions