DNA fragmentation was assessed using the Click-iT Plus TUNEL Assay Kit (ThermoFisher) according to the manufacturers protocol, and quantification was performed on liver sections centered on the central vein, where TUNEL-positive and -negative nuclei were manually counted across the full field
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Certain conditions are contraindicated in the use of vitamin D3
showed that CNS administration of the MC4R antagonist, SHU9119, completely blocked leptin-induced increase in BAT UCP1 mRNA expression [38]
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Its characteristics include cell swelling and the release of cytoplasmic content and inflammatory factors, initiating an inflammatory cascade that leads to cell damage [103, 104]