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Cells were rinsed twice with pre-warmed PBS and re-suspended in 100 l PBS containing 2 M ThiolTracker TM violet dye and incubated for 15 min at 37C

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However, the effect tended to diminish over time, so it might be a good strategy to use it over short cutting cycles [13]

GLP-1 medications should not be taken by anyone with a personal history or family history of medullary thyroid cancer, as development of these tumors were noted during early animal studies

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