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An in-vitro study found that berberine protected NSC34 cells from H 2 O 2 -induced cytotoxicity by reducing ROS production, restoring glutathione, and superoxide dismutase activity, and activating the production of antioxidant proteins nuclear factor erythroid 2-related factor-2 (Nrf2) and HO-1 (Hsu et al., 2012

Brzoska T

Glutathione reduces oxidative stress and blocks the production of melanin

Switala J, Loewen PC

SIRT3 deacetylates ceramide synthases: implications for mitochondrial dysfunction and brain injury

First, 0.4 mg/ml TGR protein was mixed with 5 mM of inhibitor 9 in buffer solution containing 0.15% DMSO and incubated 30 min at room temperature (20 C)