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Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice

Potential issues if used in individuals with asthma or certain liver enzyme deficiencies

Cytotoxicity in mammalian cells Vero cells (African Green Monkey Kidney cells, ATCC CCL-81) were grown in Dulbeccos modified Eagles medium (DMEM) containing glucose, L-glutamine and sodium pyruvate and supplemented with 10% fetal bovine serum and 1X penicillinstreptomycin (Sigma) at 37 C in culture flasks (TPT-90025) until confluent growth was attained

Sample processing for metabolomics Frozen liver and kidney samples were weighed and transferred to 1.5 mL Safelock Eppendorf tubes

In just over half the cases, users substantially exceeded dosing limits