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Article Snippet: Purifed PBMCs, at a density of 1.5 106 cells/mL, were seeded in a fask (Corning, 430,168) coated with scFvCD16A-sc4-1BBL (2 g/ml, 24 h at 4 C), and cultured in KBM-581 medium (Corning, 8851-CM) containing 5% heat-inactivated autologous plasma and 1000 IU/mL rhIL-2 (Jinsili, Jiangsu, China) for 13 days at 5% CO2 and 37, by adding fresh medium every 23 days to maintain the cell density between 1.3 and 3.2 106 cells/mL. Li, Y., Xie, S., Chen, M

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doi: 10.4162/nrp.2010.4.2.142 28