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A 2030 g of total protein were resolved on 412% NuPAGE BisTris gels (Thermofisher) and transferred to nitrocellulose membrane using a semi-dry iBlot2 kit (Thermofisher)

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Cellular GSSG accumulation, as opposed to GSH reduction, rendered hepatocytes susceptible to TNF cytotoxicity Increased GSSG/GSG ratios in response to H 2 O 2 exposure can stem from both GSH reduction and GSSG accumulation (data not shown)

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