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These were cultured with Dulbeccos modified Eagles medium (D5796, Sigma-Aldrich, Saint-Louis, MO) supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich), 100 U/mL penicillin, and 100 g/mL streptomycin (Nacalai Tesque)

Zinc is an essential mineral for immune function, wound healing, protein synthesis, and cell division, and boron is a trace mineral that plays a key role in bone growth and wound healing

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in addition, very low concentrations (0.0010.01 g/ml) corresponding to environmental exposure also increased ROS production, which induced eryptosis due to oxidative stress

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