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A critical component was the establishment of an in vitro co-culture system using 12-well plates and 0.4 m pore size transwell inserts

Wu J, Liu DJ, Shou XJ, Zhang JS, Meng FC, Liu YQ, Han SP, Zhang R et al (2018) Chinese children with autism: a multiple chemical elements profile in erythrocytes

IF and IHC staining of tissue sections Tumour tissue sections were deparaffinized in Histo-Clear (Scientific Laboratory Supplies) for 5 min, rehydrated through a series of decreasing ethanol concentrations, followed by a final 5 min wash in distilled water (dH 2 O)

once punctured, refrigerate at 28C and use within 28 days

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