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It is used to map pathway outputs such as cAMP/PKA signaling, ERK phosphorylation, immediate early gene expression, and downstream transcriptional programs

Relative mRNA expression was calculated by Ct method

Several amino acids had their concentrations increased following MTX treatment: glycine, alanine, phenylalanine, threonine, histidine, valine, arginine, leucine, isoleucine, tyrosine, and proline

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