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Previous clinical trials with GLP-1 receptor agonists are outlined in Table 2

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However, in A Diabetes Outcome Progression Trial (ADOPT), metformin exhibited a reduced risk of monotherapy failure compared with the sulfonylurea glibenclamide (known as glyburide in the USA and Canada) and slower rate of loss of beta cell function, although it was inferior to the thiazolidinedione rosiglitazone [20]

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Heart slices were then incubated with 1% 2,3,5-triphenyltetrazolium chloride (TTC) in Tris buffer (pH 7.4) for 15 min at 37 C and fixed in 4% formalin for 24 h