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Importantly, behavioral gains were observed to regress after discontinuation of treatment, indicating that ongoing supplementation may be necessary to sustain benefits

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Primary cultures of satellite cells were prepared from the upper hindlimb and back muscles of 910 month-old male rats and cultured for 24 h beginning at 24-h post-plating in DMEM-10% HS containing 5 ng/ml HGF that was pre-treated with GSSSG and LASSS separately at 1:4000 molar ratio just prior to peroxynitrite treatment (1:2000 to HGF

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To directly determine whether this hyperphosphorylation is CDK11-dependent, we first depleted SNIP1 to induce SF3B1 phosphorylation and subsequently treated cells with OTS964 (Fig