Superoxide anions are generated mainly by NOX, and although relatively weak oxidising agents, they are rapidly converted to more damaging ROS species, such as the hydroxyl radical and H 2 O 2 , or the highly reactive peroxynitrite radicals formed when in the presence of nitric oxide (NO) [30]
Tetramethyl ethylene diamine (100 L, 100%, TEMED, Acros) was added and vortexing continued for another minute
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Nutrients are degraded by harsh acid
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