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The liver tissues were digested with 5% KOH at 37 C overnight and washed twice with PBS

Pernicious anemia occurs more commonly in people who already have diseases that are linked to immune-system abnormalities, such as Graves disease, hypothyroidism (under-functioning thyroid gland), thyroiditis (inflammation of the thyroid), vitiligo, and Addison's disease (adrenocortical insufficiency)

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"AngIV-analog dihexa rescues cognitive impairment and recovers memory in the APP/PS1 mouse via the PI3K/AKT signaling pathway."Brain sciences11.11 (2021): 1487

Therefore, pairing copper with niacinamide appears relevant, as niacinamide helps protect the skin barrier and defends the skin against oxidative stress