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Then, prepared cell lysates were plated on a 96-well plate (Nunc) overnight at 4 C and incubated with 5% BSA PBST (NaCl 137 mM, KCl 2.7 mM, Na 2 HPO 4 10 mM, KH 2 PO 4 1.8 mM, 0.1% Tween 20) for 2 h at room temperature (RT)

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Results showed that, without GSH, the type strain exhibited high levels MICs (32 g/mL) for all tested -lactam antibiotics and MICs of 8 g/mL (chloramphenicol), 32 g/mL (ciprofloxacin), 256 g/mL (gentamicin), 32 g/mL (tetracycline), and 4 g/mL (vancomycin)

Brownfoot F, Rolnik DL (2024) Prevention of preeclampsia

Does BPC-157 need refrigeration

This assay uses Ellman's method, in which DTNB reacts with the reduced glutathione generated from the reduction of oxidised glutathione to form a yellow product