Immunoprecipitation was then performed using the Catch and Release Immunoprecipitation Kit (EMD Millipore), according to the manufacturers instructions with 250 g of total protein from cells or 400 g from tissues, and 1 g of an anti-IGF-1R antibody (#9750, Cell Signaling)
Am J Clin Nutr 2000 , 72 , 618S-623S [2] Rebouche, C.J
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