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For prevalent flavonoids like isorhamnetin and baicalin, their known potential as pan-assay interfering substances (PAINS) raises concerns about the specificity of reported multi-target effects, which may represent assay artifacts rather than genuine therapeutic mechanisms

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The decrease in SOD2 in OA chondrocytes is related to the increase in promoter methylation (Scott et al., 2010)

Additionally, some existing drugs not originally aimed at the microbiome turn out to have microbiome-mediated effects

The elevated levels of 2-oxoisocaproic acid indicate enhanced leucine degradation, suggesting a shift towards energy production pathways to support the heightened metabolic activity 36