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Cell debris was removed by centrifugation at 4400 g at 4 C for 30 min and the clarified cell lysate was used for protein purification

We prove that the sorting system works efficiently in both the adherent Lenti-X 293T and suspension K-562 cells to enrich positive cells to an extent comparable to or greater than the present FACS and MACS methods ( Our cell sorting system has several advantages compared to the existing transfection-positive cell sorting methods, including FACS, drug screening, and MACS

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