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The integrated density of the blots was determined by the software ImageJ, and normalized to that of -actin

10.3349/ymj.2016.57.4.865 Yonsei Med

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The World Journal of Mens Health, 37 (1), 19-30

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Hence, the concept of the gut-X axis is represented by the gutbrain axis (12), which refers to the dual communication between the gut and brain via a neuroendocrine pathway, externally impacting mood, cognition, and behavior, and vice versa