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We begin with a thorough evaluation, including appropriate laboratory testing, to confirm whether a deficiency or absorption issue is present

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Detection of Cellular ROS Levels Intracellular and mitochondrial ROS levels were detected using fluorescent measurement assay by the uptake of 1 M DCFH-DA (Beyotime, China) and 5 M MitoSOX Red (Thermo Scientific, USA), respectively

Reduction percentage for every patient was calculated according to this equation: Grading of reduction percentage of Hemi-MASI score was performed according to Khunger and colleagues as follows

Vaginal smears were taken from each animal 5 minutes before surgery on days 9, 14, and 28 of the experiment and Pap stained for cytological evaluation