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Briefly, 2 days after confluence (Day 0), the cells were placed in differentiation medium consisting of DMEM, 10% FBS, 0.5 mmol/L IBMX (MCE, USA), 1 mol/L DEX (MCE, USA), and 10 g/mL INS (MCE, USA) and then switched to maintenance medium supplemented with DMEM, 10% FBS and 10 g/mL INS

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While acetaminophen is often recommended as first-line treatment for chronic pain conditions like osteoarthritis in older adults, its use requires careful consideration