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The final measurement at the end of this step reflects the total content of GSH (free GSH + GSSG) in the samples, to calculate the GSSG content, the free GSH content must be subtracted from the total GSH content

There is sufficient evidence that in inflammatory states like HIV and diabetes, excessive ROS can deplete GSH and reduce macrophage productivity

Elkanzi NAA, Hrichi H, Alolayan RA et al (2022) Synthesis of chalcones derivatives and their biological activities: a review

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