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During these check-ups, healthcare providers may conduct blood tests to assess liver enzyme levels, which can indicate inflammation or damage

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The heterologous expression of the five mutants Phe260Gly, Asn240Lys, Leu244Arg, Asn240Lys/Leu244Arg and Asn240Thr/Leu244Arg yielded adequate amounts of soluble enzyme

RNA isolation, cDNA synthesis and quantitative PCR in HepG2 cells For RNA isolation 50,000 cells (HepG2 and HepG2 ATP7B-KO) were plated in 800 l Medium in a 24-well-plate for 24 h