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33,34 RNA splicing is a crucial step in gene expression, and abnormalities in this process are the main cause of genetic variations and diseases

and amplified by PCR

Specifically, 8 of the 11 sites are fully identical over the 10 residues spanning the P5 to P5 positions, and only two of the 11 cleavage sites have more than a single amino acid difference within the 10 residue span (Figure S1)