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The Cmax values for the gel treatment groups were 2200 290 ng/mL (PNP-1-10-0.05-TZP) ( Figure 4B , Table S2 ) and 3500 1200 ng/mL (PNP-1-10-0.05-Sema) ( Figure 4B , Table S2 )

After centrifugation (1200 g , 4 C, 10 min) 9 mL acidified H 2 O was added (final pH = 3.5) and samples were subjected to solid phase extraction

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So nutrients that are exposed to the distal gut will provoke a great response with not only GLP-1 but also other hormones like PYY, which subsequently feeds back to slow gastric emptying and retard small intestinal transit