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Some of this was expected, since insulin is a vasodilator, but the ligand also spread more widely into hypothalamic tissue, suggesting insulin was actively promoting transport into the brain

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Quantitative RT-PCR RNA was extracted from cell lysates using RNeasy Mini Kit (Qiagen, Chatsworth, CA, USA) and was reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen, Chatsworth, CA, USA)

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