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Due to the inability to effectively produce cytokines and exert antiviral activity when CD8 + T cells are exhausted during acute immune activity and are re-exposed to HBV, chronic replication of HBV and the occurrence of HCC may result [45]

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Injections may cause redness, swelling, or mild irritation at the site of administration

Before experiments, fish were acclimated in 100 cm 45 cm 45 cm tanks for 1 week and fed with commercial zebrafish diet (protein 50, lipid 8%) (Shengsuo Co., Shandong, China)

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