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-The use of the CA inhibitor ACTZ in the experiments presented in Figure 3,, needs a better introduction -- it should be made clear to the reader how CA inhibition will affect the Cl gradients

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We transfected primary human peripheral blood macrophages with wild-type and K689R NLRP3 mutant plasmids, and measured the half-life of ectopically expressed NLRP3 protein with or without CSE exposure